Fig. 4
Restoration of KMT2C expression decreases growth of RKO cells. a Growth curve for RKO KMT2CinsG clones (KI1-2). Cell confluency was tracked by daily imaging for 1 week. Bars represent standard deviation for three replicate wells per sample (KI1, P < 0.05 and KI2 P < 0.01 for time points 48–168 h (Student’s t-test)). b Colony formation assay showing ability to form colonies from single cells for RKO KMT2CinsG clones. Triplicate sample wells were normalized to the average relative number of colonies for parental RKO cells. c Growth curve for HCT116 KMT2CinsG clones (KI1-3). Cell confluency was traced by daily imaging over the course of 5 days. Bars represent standard deviation for three replicate wells per sample. d Colony formation assay for HCT116 parental cells and KMT2CinsG clones. e Ability for anchorage independent-growth measured through the soft agar assay for HCT116 parental cells and KMT2CinsG clones. For d–e, all wells were normalized to the average for the respective parental HCT116 cells for each plate, and the average relative number of colonies from two independent experiments is shown for each cell line. Error bars, SD. Two tailed Student’s t-test; *P < 0.05, ***P < 0.001