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Fig. 3 | Clinical Epigenetics

Fig. 3

From: Restoration of KMT2C/MLL3 in human colorectal cancer cells reinforces genome-wide H3K4me1 profiles and influences cell growth and gene expression

Fig. 3

Restored KMT2C expression increases H3K4me1 at enhancers of upregulated genes in RKO and HCT116 cells. a–b ChIP-seq detection of H3K4me1 enriched regions at different stringencies of peak detection in RKO KMT2CinsG KI2 clone (a) and HCT116 KMT2CinsG KI1 and KI2 clones (b). c–d H3K4me1 enrichment at global human genome enhancers and at enhancers of upregulated genes in (c) RKO parental and KMT2CinsG KI2 clone and (d) HCT116 parental and KMT2CinsG clones KI1 and KI2 (***P = < 0.001). Dashed lines mark the median of the parental cells in c–d. e H3K4me1 signal at the CCD80 gene, which was upregulated in RKO KMT2CinsG KI2 cells. f Detection of H3K4me1 at the chromosomal region of the ARL4C gene which was upregulated in HCT116 KMT2CinsG KI1 and KI2 clones. Enhancer-promoter associations are marked by dotted lines in e–f

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