Fig. 4

Transgenerational changes in IFN- enprotein levels in the spleen after alcohol feeding in pregnant female rats. IFN-ϒ protein levels in the spleens of F2 and F3 male (a, c) and female (b, d) rat offspring from different germ lines as defined in Fig. 2a. IFN-ɣ protein levels in the spleens were measured by western blotting. Representative blots for IFN-ϒ, β-actin were shown in the upper panel and quantification data normalized with β-actin were represented as a histogram in the lower panel for each figure. Data are mean + SEM (n = 6–7) and were analyzed using one-way ANOVA with the Newman-Keuls post-hoc test. * P < 0.05, ** P < 0.01 between AFM vs. AD or PFM. F statistics and P values of data shown in figures were presented in Supplementary Table 1