Fig. 2

Transgenerational changes in Ifn-ɣ mRNA expression in the spleen after alcohol feeding in pregnant female rats. A schematic diagram to indicate how F2 and F3 male germ line (AFM) and female germ line (AFF) of fetal alcohol-exposed (a) and male germ line (PFM) and female germ line (PFF) of pair-fed control offspring (b) were generated. Ifn- mRNA levels in the spleen tissues of F2 (c, d) and F3 (e, f) male and female rat offspring from different germ lines were measured by quantitative RT-PCR. Data are mean + SEM (n = 8) and were analyzed using one-way ANOVA with Newman-Keuls post-hoc test; ** P < 0.01, *** P < 0.001 between AFM and controls (AD, PFM). F statistics and P values of data shown in figures were presented in Supplementary Table 1