Fig. 3

Adipogenesis changes in stably transfected ABCG1 KD cells. a Western blot protein expression of ABCG1 a during differentiation b after silencing in SGBS cell lines. c Lipid content analysed by red oil of ABCG1 KD compared to controls. d Expression of key adipose genes at day 12 differentiation in KD ABCG1 cells compared to shRNA controls, normalised to housekeeping gene B2M and compared to DMSO-vehicle controls. Experiments were performed at n = 4 biological replicates. e Glucose uptake in ABCG1 KD compared to controls stimulated with or without 1 μM insulin for 1 h. Fold change in KD and control cells compared to cells not treated with insulin. f Analysis of insulin signalling in SGBS ABCG1 KD cell line through protein expression of phosphorylated AKT at day 12, stimulated with or without 200 nM insulin for 1 h, using western blot analysis. *p < 0.05; **p < 0.001; ***p < 0.0001