Fig. 2

TMEM240 may repress cell growth, migration, and cell cycle arrest in colon cancer cells. a A recombinant pMyc-DDK-hTMEM240 plasmid was transfected into DLD-1 cells for 24 h, and then the cells were analyzed via real-time RT-PCR for mRNA and Western blotting for protein (left). The cell proliferation rate was analyzed by sulforhodamine B (SRB) assay (middle). A bright view was taken to illustrate the cell morphology (right) (original magnification, × 100). b si-TMEM240 was transfected into DLD-1 cells. The mRNA expression (up), cell proliferation rate (down), and cell morphology (right) of DLD-1 cells were analyzed (original magnification, × 100). c The mRNA expression (up), cell morphology (right), and cell proliferation rate (down) in HCT116 colon cancer cells were analyzed (original magnification, × 40). d The migratory ability was measured by a transwell assay after TMEM240 overexpression in DLD-1 cells. e A TMEM240 and/or si-TMEM240 plasmid was transfected into DLD-1 cells for 24 h, and then the cell cycle distribution was analyzed by flow cytometry. Data are presented as the mean ± SD, *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001. A t test was used to calculate group differences in all experiments. Experiments were performed with at least two biological duplicates and three technical replicates