Fig. 1
Prostate cancer-associated fibroblasts have distinctive changes in DNA methylation. a Schematic of the cohort of patient-derived fibroblasts analysed with EPIC arrays. Asterisks denote that WGBS data was available for three pairs of CAFs and NPFs. b MDS plot of the 1000 most variably methylated CpGs in EPIC array data showing clear separation of CAFs from NPFs and BPFs in patients 4–17; however, CAF18 clustered with NPFs and BPFs. c Volcano plot of differentially methylated positions (DMPs) in CAFs versus NPFs (patients 4–17). DMPs are shown in orange, while other probes are in blue. For all volcano plots, dotted lines indicate > 10% change in methylation and −log10 adjusted P value > 1 (adjusted p value > 0.1). d Dendrogram and heat map from unsupervised hierarchical clustering of the EPIC CAF-DMRs showing clear separation of CAFs from NPFs and BPFs. e and f Volcano plots of DMPs in CAFs versus BPFs and NPFs versus BPFs. DMPs from CAFs versus NPFs (panel c) are shown in orange. g Venn diagram showing the overlap between DMPs in CAFs versus NPFs compared to CAFs versus BPFs