Fig. 6From: Crosstalk between DNA methylation and histone acetylation triggers GDNF high transcription in glioblastoma cellsCBP significantly increases histone H3 acetylation in GDNF promoter II and RNA polymerase II recruitment at the TSS in GBM cells. a Binding of CBP to the CRE of GDNF enhancer II and the TSS in GBM and NB tissues (n = 3). b Histone H3 acetylation in the CRE of GDNF enhancer II and at the TSS in GBM and NB tissues (n = 3). c RNA polymerase II recruitment to the CRE of GDNF enhancer II and the TSS in GBM and NB tissues (n = 3). d Binding of CBP to the CRE of GDNF enhancer II and the TSS in U251 and NHA cells (n = 3). e Histone H3 acetylation in the CRE of GDNF enhancer II and at the TSS in U251 and NHA cells (n = 3). f RNA polymerase II recruitment to the CRE of GDNF enhancer II and the TSS in U251 and NHA cells (n = 3). g Comparison of mRNA levels (log2 median-centered ratio) of human CBP in NB samples (n = 10) and GBM brain samples (n = 542) from TCGA brain dataset analyzed on the Oncomine® Platform. h CBP protein expression in NHA and U251 cells and GBM tissue as determined by western blot (n = 3). iCBP mRNA expression after CBP knockdown in U251 cells as determined by real-time PCR (n = 3). j CBP protein expression after CBP knockdown in U251 cells as determined by western blot (n = 3). k Histone H3 acetylation in the CRE of GDNF enhancer II and at the TSS after CBP knockdown in U251 cells as determined by ChIP-PCR (n = 3). l RNA polymerase II recruitment to the CRE of GDNF enhancer II and the TSS after CBP knockdown as determined by ChIP-PCR (n = 3). mGDNF mRNA expression after CBP knockdown in U251 cells as determined by real-time PCR. RNA POL II indicates RNA polymerase II (n = 3). All data are mean ± SD. *P < 0.05; **P < 0.01Back to article page