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Fig. 6 | Clinical Epigenetics

Fig. 6

From: Crosstalk between DNA methylation and histone acetylation triggers GDNF high transcription in glioblastoma cells

Fig. 6

CBP significantly increases histone H3 acetylation in GDNF promoter II and RNA polymerase II recruitment at the TSS in GBM cells. a Binding of CBP to the CRE of GDNF enhancer II and the TSS in GBM and NB tissues (n = 3). b Histone H3 acetylation in the CRE of GDNF enhancer II and at the TSS in GBM and NB tissues (n = 3). c RNA polymerase II recruitment to the CRE of GDNF enhancer II and the TSS in GBM and NB tissues (n = 3). d Binding of CBP to the CRE of GDNF enhancer II and the TSS in U251 and NHA cells (n = 3). e Histone H3 acetylation in the CRE of GDNF enhancer II and at the TSS in U251 and NHA cells (n = 3). f RNA polymerase II recruitment to the CRE of GDNF enhancer II and the TSS in U251 and NHA cells (n = 3). g Comparison of mRNA levels (log2 median-centered ratio) of human CBP in NB samples (n = 10) and GBM brain samples (n = 542) from TCGA brain dataset analyzed on the Oncomine® Platform. h CBP protein expression in NHA and U251 cells and GBM tissue as determined by western blot (n = 3). iCBP mRNA expression after CBP knockdown in U251 cells as determined by real-time PCR (n = 3). j CBP protein expression after CBP knockdown in U251 cells as determined by western blot (n = 3). k Histone H3 acetylation in the CRE of GDNF enhancer II and at the TSS after CBP knockdown in U251 cells as determined by ChIP-PCR (n = 3). l RNA polymerase II recruitment to the CRE of GDNF enhancer II and the TSS after CBP knockdown as determined by ChIP-PCR (n = 3). mGDNF mRNA expression after CBP knockdown in U251 cells as determined by real-time PCR. RNA POL II indicates RNA polymerase II (n = 3). All data are mean ± SD. *P < 0.05; **P < 0.01

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