PCR | Primer | Sequence (5′-3′) | Product size (bp) | PCR cycles | Annealing temperature (°C) |
---|---|---|---|---|---|
qRT-PCR | GAPDHF | GGAGTCAACGGATTTGGT | 206 | 23 | 55 |
GAPDHR | GTGATGGGATTTCCATTGAT | ||||
SEPT9_v2F | TGGGATCATTTCGGACTTCG | 167 | 60 | ||
SEPT9_v2R | AGGGTTCTGAGTTCTTCACG | ||||
MMP1F | GAGATCATCGGGACAACTCTCCTT | 119 | |||
MMP1R | GTTGGTCCACCTTTCATCTTCATCA | ||||
MMP10F | AGACACTGACACTCTGGAGG | 202 | |||
MMP10R | TGGAGTCACCTCTTCCCAGA | ||||
FZD10F | ATCGGCACTTCCTTCATCCTGTC | 199 | |||
FZD10R | TCTTCCAGTAGTCCATGTTGAG | ||||
miR-92b-3pF | TATTGCACTCGTCCCG | ||||
miR-92b-3PR | GTGCAGGGTCCGAGGT | ||||
miR-92b-3PRT | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGGAGGC | ||||
U6F | CTCGCTTCGGCAGCACA | ||||
U6R | AACGCTTCACGAATTTGCGT | ||||
U6RT | AACGCTTCACGAATTTGCGT | ||||
GAPDHF | CCAGCAAGAGCACAAGAGGAA | 114 | |||
GAPDHR | CAAGGGGTCTACATGGCAACT | ||||
MSP | SEPT9_v2m1 | GGTTTAGGGGTTTTTTCGGC | 137 | 60 | |
SEPT9_v2m2 | AACTAATAAACAACGAATCGCG | ||||
SEPT9_v2u1 | GGGTTTAGGGGTTTTTTTGGT | 141 | 58 | ||
SEPT9_v2u2 | AATAACTAATAAACAACAAATCACA |