Skip to main content

Table 2 List of primers used in this study

From: SEPT9_v2, frequently silenced by promoter hypermethylation, exerts anti-tumor functions through inactivation of Wnt/β-catenin signaling pathway via miR92b-3p/FZD10 in nasopharyngeal carcinoma cells

PCR

Primer

Sequence (5′-3′)

Product size (bp)

PCR cycles

Annealing temperature (°C)

qRT-PCR

GAPDHF

GGAGTCAACGGATTTGGT

206

23

55

GAPDHR

GTGATGGGATTTCCATTGAT

   

SEPT9_v2F

TGGGATCATTTCGGACTTCG

167

 

60

SEPT9_v2R

AGGGTTCTGAGTTCTTCACG

   

MMP1F

GAGATCATCGGGACAACTCTCCTT

119

  

MMP1R

GTTGGTCCACCTTTCATCTTCATCA

   

MMP10F

AGACACTGACACTCTGGAGG

202

  

MMP10R

TGGAGTCACCTCTTCCCAGA

   

FZD10F

ATCGGCACTTCCTTCATCCTGTC

199

  

FZD10R

TCTTCCAGTAGTCCATGTTGAG

   

miR-92b-3pF

TATTGCACTCGTCCCG

   

miR-92b-3PR

GTGCAGGGTCCGAGGT

   

miR-92b-3PRT

GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGGAGGC

   

U6F

CTCGCTTCGGCAGCACA

   

U6R

AACGCTTCACGAATTTGCGT

   

U6RT

AACGCTTCACGAATTTGCGT

   

GAPDHF

CCAGCAAGAGCACAAGAGGAA

114

  

GAPDHR

CAAGGGGTCTACATGGCAACT

   

MSP

SEPT9_v2m1

GGTTTAGGGGTTTTTTCGGC

137

 

60

SEPT9_v2m2

AACTAATAAACAACGAATCGCG

   

SEPT9_v2u1

GGGTTTAGGGGTTTTTTTGGT

141

 

58

SEPT9_v2u2

AATAACTAATAAACAACAAATCACA