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Fig. 5 | Clinical Epigenetics

Fig. 5

From: SEPT9_v2, frequently silenced by promoter hypermethylation, exerts anti-tumor functions through inactivation of Wnt/β-catenin signaling pathway via miR92b-3p/FZD10 in nasopharyngeal carcinoma cells

Fig. 5

SEPT9_v2 inactivated the Wnt/β-catenin signaling pathway by downregulating FZD10. a FZD10 mRNA expression was detected by qPCR. Values are presented as the mean ± SD of at least three independent experiments. b TOP/FOP Flash reporter assays were performed to test the regulation of SEPT9_v2 on the Wnt/β-catenin pathway in stably transfected HONE1 cells, HNE1 cells, and 293T cells. Values are presented as the mean ± SD of at least three independent experiments. c Western blots were performed to detected β-catenin and the downstream Wnt/β-catenin genes. d The expression of SEPT9_v2 and β-catenin were detected by immunofluorescence staining in stably transfected HONE1 and HNE1 cells. SD, standard deviation. The nuclei were counterstained with DAPI. DAPI, 4,6-diamidino-2-phenylindole. *p < 0.05, **p < 0.01, ***p < 0.001

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