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Fig. 2 | Clinical Epigenetics

Fig. 2

From: SEPT9_v2, frequently silenced by promoter hypermethylation, exerts anti-tumor functions through inactivation of Wnt/β-catenin signaling pathway via miR92b-3p/FZD10 in nasopharyngeal carcinoma cells

Fig. 2

Ectopic SEPT9_v2 inhibited NPC cell proliferation and colony formation by inducing G0/G1 cell cycle arrest and apoptosis. HONE1 and HNE1 cells stably transfected with SEPT9_v2 or vector (pcDNA3.1) were used. a SEPT9_v2 mRNA expression was detected in HONE1 and HNE1 cells stably transfected with SEPT9_v2 or vector (pcDNA3.1) by RT-PCR and Western blot. FLAG-tag antibody was used to detect SEPT9_v2 protein. GAPDH was used as an input control. b The ability of cell proliferation was measured in stably transfected cells by MTS assay. c The stably transfected cells were used for colony formation assay to detect proliferation rates. Left: representative images. Right: histogram statistics of the colony formation assay. d The number of cells in the G0/G1 phase was detected in HONE1 and HNE1 cell lines stably transfected with SEPT9_v2 or vector. Left: representative flow cytometry plots. Right: histogram statistics of the distribution of cell population. e The proportion of apoptotic cells in transiently transfected HONE1 and HNE1 cell lines. Left: representative flow cytometry plots. Right: quantification of apoptosis changes. All values are presented as the mean ± SD of at least three independent experiments. SD, standard deviation. *p < 0.05, **p < 0.01, ***p < 0.001

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Clinical Epigenetics

ISSN: 1868-7083

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