Underestimated effect of intragenic HIV-1 DNA methylation on viral transcription in infected individuals

Clinical Epigenetics

Table 2 Performance of the nine final assays compared with the predicted performance using in silico analysis of the primer complementarity

	LTR	NCR	ENV	ETR
# primer combinations	2	3	1	3
% of patients expected to generate amplicons based on in silico analysis^*	59.25	80.37	89.42	60.33
% of patients in which DNA was amplified	51.40	81.94	100	63.90
% of patients for which the DNA had sufficient quality to be mapped to HIV-1	48.61	75.00	41.67	63.90

^*In silico analysis is based on the bioinformatics primer evaluation tool as described by Rutsaert et al. [46]

ISSN: 1868-7083