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Fig. 4 | Clinical Epigenetics

Fig. 4

From: CHD4/NuRD complex regulates complement gene expression and correlates with CD8 T cell infiltration in human hepatocellular carcinoma

Fig. 4

Knockdown of CHD4 inhibits HCC cell migration and invasion. a, b Wound healing assay was performed to examine the effect of CHD4 knockdown on HCC cell migration. c, d Transwell invasion assay was performed to examine the effect of CHD4 knockdown on HCC cell invasion. e, f Expression levels of the EMT marker genes were detected in CHD4 knockdown cell lines and negative control cell lines by western blotting analysis and immunofluorescence staining. g Western blotting assay to examine the effects of CHD4 knockdown on histone modifications. h ChIP assay to examine the effects of CHD4 knockdown on histone modifications at the promoter of the CTNNB1 and Vimentin genes. *p < 0.05, **p < 0.01, ***p < 0.001, unpaired t test

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