Fig. 3

Knockdown of CHD4 inhibits proliferation and colony-forming ability and increases apoptosis of HCC cells. a The expression levels of CHD4 were detected in CHD4 knockdown cell lines and negative control cell lines using western blotting analyses. β-Tubulin was used as loading control. b CCK-8 assay was performed to assess the effect of CHD4 knockdown on HCC cell proliferation. c Clonogenic assay was performed to examine the effect of CHD4 knockdown on colony-forming ability of HCC cells. d The effect of CHD4 knockdown on apoptosis of HCC cells was examined by flow cytometry analysis. *p < 0.05, **p < 0.01, ***p < 0.001, unpaired t test