Fig. 5
TET1 and TET2 are repressed in conditional Braf-V637E knock-in mice. aTET1, TET2, and Mlh1 mRNA expression in the proximal small intestine of mutant BrafV637E mice (n = 13) relative to wild-type BrafWT mice (n = 5). Shown are the median (line) and mean (red circle). P values were calculated with Welch two sample t-test. b Differential methylated probes either hypermethylated (red) or hypomethylated (green) in BrafV637E mice versus BrafWT mice. Numbers of probes are at the top and number of samples are shown as n. c Experimental set up underlying samples used for genome-wide methylation analysis on mouse tilling array (NimbleGen) taken from mice either at less than 13-week (< 13 weeks) or more than 55-week (> 55 weeks) timepoint (upper). Box plots showing average DNA methylation at hypermethylated probes (744) in BrafWT and BrafV637E mice at < 13- or > 55-week timepoints. Plotted are the input-normalized intensity levels on y-axis (log2) with medians (line) and means (red circle). P values were calculated with Wilcoxon rank-sum test. d Scatter plot showing methylation in BrafV637E mice at < 13 or > 55-week timepoint. Plotted are the input-normalized intensity levels (log2) of hypomethylated (green) or hypermethylated (red) DMPs. The 346 probes methylated at > 55-week timepoint are indicated in the box. P values were calculated with Wilcoxon rank-sum test