Fig. 5

miR-484 suppresses MMP14 and HNF1A expression by targeting their 3′UTRs in CC cells. a The diagram shows the potential target genes (overlapping fraction) that were shared by all three databases and the technical route of selecting candidate targets. b The predicted miR-484-binding sites in HNF1A and MMP14 mRNA using Targetscan 7.0 are shown. c RT-qPCR showing HNF1A and MMP14 mRNA levels after transfection with pri-miR-484 or ASO-miR-484. d HeLa and C33A cells were co-transfected with wild-type pcDNA3/EGFP-MMP14 3′UTR or 3′UTR-mut and pri-miR-484 or ASO-miR-484. e HeLa and C33A cells were co-transfected with wild-type pcDNA3/EGFP-HNF1A 3′UTR or 3′UTR-mut and pri-miR-484 or ASO-miR-484. f RT-qPCR showing the expression of HNF1A and MMP14 in 20 pairs of human cervical cancer tissues and the adjacent non-cancerous tissues. U6 snRNA was used as the internal control. g Pearson’s correlation analysis indicated the negative correlation between the expression of miR-484 and HNF1A (r = − 0.64**) and MMP14 (r = − 0.68**). All of the experiments were repeated three times. *p < 0.05, **p < 0.01, ***p < 0.001; NS, not significant