Fig. 4
miR-484 suppresses CC cell adhesion in vitro and metastasis in vivo. a Cell–matrix adhesion assay of HeLa cells adhering to FN and Matrigel (M) with or without RGDfv for 30, 60, and 90 min, respectively. Representative images under microscope were showed (× 100). b, c Quantitative results of three independent experiments of cell–matrix adhesion assay in HeLa (b) and C33A cells (c). The adherent cells were measured by software of ImageJ (*p < 0.05, **p < 0.01). d Cell–cell adhesion assay of HeLa cells suspension cultured with or without RGDfv for 24 h. Left: representative images, the photomicrographs were taken at × 100 magnification. Right: quantitative results of three independent experiments (*p < 0.05, **p < 0.01). e Western blot results showing β1-integrin, HNF1A, and MMP14 expression in HeLa cells after transfection with pri-miR-484 or ASO-miR-484. The expression levels were normalized to GAPDH (*p < 0.05, **p < 0.01). f A nude mouse tumor xenograft model was utilized to study the effect of miR-484 on tumor growth in vivo. The mice were euthanized, and the tumors were isolated 20 days after implantation. The miR-484 level in tumors was shown on the right of the picture. g The tumor size was measured 8 days after injection every other day; the tumor growth curve is shown. The tumor volume = length × width2 × 1/2, *p < 0.05, **p < 0.01, n = 6 (Student’s t test)