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Fig. 6 | Clinical Epigenetics

Fig. 6

From: ZMYND10, an epigenetically regulated tumor suppressor, exerts tumor-suppressive functions via miR145-5p/NEDD9 axis in breast cancer

Fig. 6

Overexpressed ZMYND10 inhibited breast cancer metastasis via decreasing the expression of NEDD9. a The mRNA expression of NEDD9 was detected by qPCR. b Luciferase assay was performed to detect the regulation of ZMYND10 on NEDD9 in vector- and ZMYND10-expressed MDA-MB231 and SK-BR-3 cells. c Immunofluorescence showed the localization and expression of NEDD9 in MDA-MB231 and SK-BR-3 cells transfected with ZMYND10 plasmid. d After MDA-MB231 and SK-BR-3 cells were overexpressed with ZMYND10, the protein levels of NEDD9, PI3K, p-PI3K, GSK-3β, p-GSK-3β, active-β-catenin, E-cadherin, vimentin, snail, Akt, and the phosphorylation levels of Akt onSer-473 were examined. β-actin was used as an inner control

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Clinical Epigenetics

ISSN: 1868-7083

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