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Fig. 4 | Clinical Epigenetics

Fig. 4

From: ZMYND10, an epigenetically regulated tumor suppressor, exerts tumor-suppressive functions via miR145-5p/NEDD9 axis in breast cancer

Fig. 4

Ectopic ZMYND10 induced G2/M cell cycle arrest and promote cell apoptosis in breast cancer cells. a Flow cytometry analysis was used to detect the effect of ZMYND10 on cell cycle in vector- and ZMYND10-expressing MDA-MB231 and SK-BR-3 cells. Left, representative flow cytometry plots; right, the histogram statistics of cell cycle changes. b Western blotting with antibodies to cell cycle proteins, including cyclin D1, p21, and p27. c The proportion of apoptotic cells was detected in vector- and ZMYND10-expressing MDA-MB231 and SK-BR-3 cells by flow cytometry analysis. Representative flow cytometry plots (left) and the histogram statistics of apoptosis changes (right). d Western blotting with antibodies to apoptosis proteins, including Bcl-2, Bax, Bcl-xL, cleaved caspase3, and cleaved-PARP

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