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Fig. 1 | Clinical Epigenetics

Fig. 1

From: Drug screening approach combines epigenetic sensitization with immunochemotherapy in cancer

Fig. 1

Pretreatment screening protocol to test the reprogramming activity of multiple compounds. The protocol is developed to flexibly test non-simultaneous drug combinations. In our experimental design, this included pretreatment with 60 epigenetic inhibitors, followed by treatment with rituximab and doxorubicin, the major constituents in the R-CHOP combination therapy. At day 0, cells are seeded on microplates precoated with the pretreatment compounds at five different concentrations. Cells are then passaged in the plate every third day using automated liquid handling, including the corresponding pretreatment compound in the culture media. After 9 days of pretreatment, cells are treated with a fixed concentration of doxorubicin and rituximab to compare the activity of the pretreatment alone (pink dose-response curve) vs. the activity in combination with the standard treatment (purple dose-response curve)

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