Table 2 Biomarker performance of each gene promoter methylation for LC, PCa and CRC detection in ccfDNA
Genes | AUC (95 % CI) | p-value | Cut-off value | Sensitivity % (95% CI) | Specificity % (95% CI) | Accuracy % (95% CI) | Positive likelihood ratio (LR+) (95% CI) | Negative likelihood ratio (LR-) (95% CI) |
|---|---|---|---|---|---|---|---|---|
Lung Cancer | ||||||||
APCme | 0.556 (0.482-0.631) | 0.136 | 7.2629 | 26 (18-36) | 85 (77-90) | 60 (53-66) | 1.7 (1.0-2.9) | 0.87 (0.76-1.00) |
FOXA1me | 0.579 (0.505-0.653) | 0.037 | 301.1682 | 38 (29-48) | 77 (69-84) | 61 (54-67) | 1.7 (1.1-2.5) | 0.80 (0.67-0.96) |
RARβ2me | 0.599 (0.525-0.674) | 0.009 | 0.3548 | 24 (16-33) | 96 (92-99) | 65 (59-71) | 6.4 (2.5-16.2) | 0.79 (0.71-0.89) |
RASSF1Ame | 0.602 (0.528-0.677) | 0.007 | 14.5588 | 25 (17-34) | 96 (91-98) | 65 (59-71) | 5.6 (2.4-13.0) | 0.79 (0.70-0.89) |
SEPT9me | 0.596 (0.522-0.671) | 0.011 | 9.1907 | 21 (13-30) | 99 (95-100) | 65 (59-71) | 14.0 (3.4-58.4) | 0.81 (0.73-0.89) |
SOX17me | 0.619 (0.546-0.693) | 0.002 | 70.0725 | 29 (21-39) | 94 (89-97) | 66 (60-72) | 5.0 (2.4-10.4) | 0.75 (0.66-0.86) |
LC panel | - | - | - | 66 (56-75) | 70 (61-77) | 68 (62-74) | 2.2 (1.6-2.9) | 0.49 (0.37-0.66) |
Prostate Cancer | ||||||||
FOXA1me | 0.719 (0.656-0.782) | <0.001 | 295.8133 | 61 (52-70) | 77 (69-84) | 70 (64-75) | 2.7 (1.9-3.8) | 0.50 (0.40-0.64) |
GSTP1me | 0.564 (0.493-0.634) | 0.078 | 36.7086 | 15 (9-22) | 98 (94-100) | 59 (52-65) | 6.7 (2.0-22.3) | 0.87 (0.80-0.94) |
HOXD3me | 0.650 (0.583-0.717) | <0.001 | 320.9365 | 80 (72-87) | 43 (34-51) | 60 (54-66) | 1.4 (1.2-1.7) | 0.47 (0.31-0.70) |
RARβ2me | 0.594 (0.524-0.664) | 0.010 | 5.2251 | 22 (15-31) | 96 (92-99) | 61 (55-67) | 6.1 (2.4-15.3) | 0.81 (0.73-0.89) |
RASSF1Ame | 0.543 (0.472-0.614) | 0.232 | 0.5627 | 13 (8-21) | 96 (91-98) | 57 (51-64) | 3.0 (1.2-7.4) | 0.91 (0.84-0.98) |
SEPT9me | 0.550 (0.479-0.621) | 0.164 | 7.7096 | 12 (6-19) | 99 (95-100) | 58 (51-64) | 7.9 (1.8-33.9) | 0.90 (0.84-0.96) |
SOX17me | 0.611 (0.542- 0.681) | 0.002 | 16.153 | 29 (21-38) | 93 (88-97) | 63 (57-69) | 4.4 (2.2-8.7) | 0.76 (0.67-0.86) |
PCa panel | - | - | - | 72 (63-80) | 72 (64-79) | 72 (66-77) | 2.6 (1.9-3.5) | 0.39 (0.29-0.53) |
Colorectal cancer | ||||||||
SEPT9me | 0.533 (0.458 - 0.608) | 0.383 | 265.2606 | 8 (4-15) | 100 (97-100) | 61 (54-67) | - | 0.92 (0.87-0.97) |
SOX17me | 0.550 (0.475 - 0.625) | 0.189 | 732.3866 | 11 (6-19) | 100 (97-100) | 62 (56-68) | - | 0.89 (0.83-0.95) |
CRC panel | - | - | --- | 12 (6-20) | 100 (97-100) | 63 (56-69) | - | 0.88 (0.82-0.95) |