Fig. 5
From: Epigenetic therapy of novel tumour suppressor ZAR1 and its cancer biomarker function
Epigenetic therapy of ZAR1 induces ZAR1 targets p21 and WEE1. a ZAR1 promoter activity is inhibited in comparison to pRLnull empty reporter construct by luciferase assay. b ZAR1 promoter activity can be epigenetically modified by the overexpression of guide RNAs targeting ZAR1 together with epigenetic activators p300, VP160, and epigenetic inhibitors EZH2 and DNMT3A by luciferase assay. c ZAR1 endogenous expression in HeLa can be reactivated by epigenetic activators p300, VP160, and TET1 targeted to ZAR1 by RNA guides by RT-PCR. d Expression of ZAR1 targets WEE1/p21 by RT-PCR is stimulated by overexpression of ZAR1 guided VP160. e, f, g Epigenetic reexpression of ZAR1 by overexpression of ZAR1 guide directed p300, VP16,0 and TET1 or empty control for 24, 48, and 72 h as well as expression dynamics of reexpressed ZAR1 targets WEE1 and p21 by ZAR1-guided epigenetic activators