Fig. 4

ZAR1 binding partners associate with known networks, zinc-finger dependent. a ZAR1wt binding partners (n = 101 in blue; compared to IP from ZAR1delZF in yellow) and b ZAR1delZF-binding partners (n = 16 in yellow, compared to IP from ZAR1wt in blue) from GFP Trap, and subsequent mass spectrometry with ≥10% relative enrichment to respective ZAR1 in comparison to a ZAR1delZF and b ZAR1wt. Asterisks indicate the only partner protein network-associated proteins that are unaffected by zinc-finger deletion. c Depiction of ZAR1wt binding partner network and d loss of network upon zinc-finger deletion. Red circles indicate overlap of ZAR1wt/ZAR1delZF partner proteins; ZAR1 in purple. e Summary of ZAR1wt and ZAR1delZF relative numbers of identified binding proteins. f GO-term analysis of ZAR1wt binding partners (n = 101) with significant enrichment. Regarding ZAR1delZF partners the GO-terms were not enriched (n = 16)