Fig. 2
From: Epigenetic therapy of novel tumour suppressor ZAR1 and its cancer biomarker function
ZAR1 blocks cell cycle progression, partially through p53. a Enrichment plots from RNA microarray of ZAR1 overexpression reveals significant association with G2M checkpoint and the p53 pathway by GSEA analysis in HCT116. b According expression of GAPDH, ZAR1, WEE1, p27, and p21 from array upon ZAR1 overexpression. c Overexpression of ZAR1 (EYFP tagged) in HCT116wt and HCT116Δp53 cells and subsequent cell cycle analysis by flow cytometry and propidium iodide staining. Gating to subG1, G0G1, S, and G2M phases is shown. d According quantification for 24 and 30 h overexpression of ZAR1 in HCT116 cells is calculated. e Altered RNA expression analysis upon ZAR1 or control reexpression is measured by quantitative RT-PCR and normalised to GAPDH for ZAR1, WEE1, p21, and p27 in HCT116wt and HCT116Δp53 (upper) as well as in HEK and HeLa (lower)