Fig. 2
From: Ovarian cancer detection by DNA methylation in cervical scrapings
Selection and verification of candidate genes. a Hierarchical clustering analysis of potential candidate genes with methylation profiles. The heatmap represents DNA methylation levels and clustering into 4 subgroups. We verified the top 10% of hypermethylated genes, in each group. If more than 5 hypermethylated genes were shown, we chose 2 or 3 genes of each subgroup and less reported in literature which listed on the right side. b and c DNA methylation levels of candidate genes were verified by quantitative methylation-specific PCR (qMSP), using DNA pooled from tissues and cervical scrapings. Each dot shows 5 specimens with the same diagnosis in a pooled DNA. TMU-A, Taipei Medical University-A ovarian tissue dataset; N, normal; OC, ovarian carcinoma