Fig. 1

Establishing new diagnoses based on methylation profiling of CNS tumors NOS, not otherwise specified; PXA, pleomorphic xanthoastrocytoma; DNET, dysembryoplastic neuroepithelial tumor; DLGNT, diffuse leptomeningeal glioneuronal tumor Methylation profiling led to a change from initial histopathological diagnosis (left) to the final integrated diagnosis (right) or to the identification of a new clinically relevant molecular subtype in 29 cases. This subset includes patients with a newly identified diagnosis [13], a resolved differential diagnosis different from the top initial diagnosis [7], or a new clinically relevant molecular subtype [9]. WHO grading changes are shown in red (upgrading) and green (downgrading).