Fig. 2

Histone methyltransferases EHMT1 and EHMT2 are upregulated in olaparib-resistant HGSOC. a Four olaparib resistant clones of PEO1-OR were analyzed by RNA-Seq. Of all enzymes involved in H3K9 methylation, EHMT1 (red squares), and KDM1B (green circles) were significantly changed in all four populations of PEO1-OR relative to PEO1. b–d RT-qPCR analysis of histone methyltransferases EHMT1 and EHMT2, and zinc-finger gene ZNF644 in PEO1 and PEO1-OR cells (mean ± SD, n = 3, unpaired t test). e Protein lysates from PEO1 and PEO1-OR cells were analyzed by immunoblot for EHMT1, EHMT2, and Actin loading control. f, g Patient-derived HGSOC ascites cells were injected intraperitoneally into NSG mice. After 21-day treatment with olaparib or vehicle control, mice were sacrificed and ascites cells were collected and analyzed by RT-qPCR for EHMT1 and EHMT2 (mRNA expression is normalized to GAPDH and plotted as mean ± SD, n = 3 technical PCR replicates, unpaired test; numbers below bars are mouse ear-tag numbers). h Protein lysates from ascites cells were analyzed by immunoblot for EHMT1, EHMT2, and Actin loading control (ear tag numbers correspond with mRNA data)