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Fig. 4 | Clinical Epigenetics

Fig. 4

From: Novel DNA methylation biomarkers show high sensitivity and specificity for blood-based detection of colorectal cancer—a clinical biomarker discovery and validation study

Fig. 4

Detection of C9orf50, KCNQ5, and CLIP4 markers in plasma (test cohort). a Methylation-specific ddPCR was performed on 4500 copies of bisulfite-converted cfDNA to detect methylated C9orf50, KCNQ5, and CLIP4 in plasma samples from CRC patients and controls in the test cohort. The total number of methylated DNA copies (sum of the three markers) are recorded on the y-axis and CRC samples are arranged by UICC stage. b–d ROC curves (right) from test of C9orf50, KCNQ5, and CLIP4 individual markers in plasma. The red dots and dashed lines indicate the sensitivity and specificity when calling samples positive if they contained any methylated DNA. e Sensitivity and specificity by the TriMeth test in plasma. f UICC stage-stratified sensitivity of TriMeth in plasm. Error bars represent 95% CI. ddPCR droplet digital PCR, CRC colorectal cancer, UICC Union for International Cancer Control, ROC receiver operating characteristics

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Clinical Epigenetics

ISSN: 1868-7083

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