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Table 3 Ct values of genes using methylation specific quantitative real-time PCR

From: Epigenome-wide base-resolution profiling of DNA methylation in chorionic villi of fetuses with Down syndrome by methyl-capture sequencing

Target gene

N (n = 10)

DS (n = 8)

p value

MSRE (−)

MSRE (+)

ΔCt

MSRE ((−)

MSRE (+)

ΔCt

CPT1B

32.73

35.31

2.58

33.35

33.55

0.19

0.033

ZNF837

35.47

37.59

2.13

36.77

36.61

0.21

0.033

C11orf95

29.62

33.74

4.12

30.02

30.74

0.72

0.006

KIAA1875

30.55

41.94

11.59

34.37

35.65

2.89

0.007

SHROOM1

30.07

34.12

4.05

29.86

30.73

0.86

0.001

CASZ1

34.00

37.83

3.27

34.33

35.19

0.85

0.008

  1. Ct cycle threshold, DS Down syndrome, N normal, MSRE methylation-specific restriction enzyme
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Clinical Epigenetics

ISSN: 1868-7083

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