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Fig. 1 | Clinical Epigenetics

Fig. 1

From: Perturbations in imprinted methylation from assisted reproductive technologies but not advanced maternal age in mouse preimplantation embryos

Fig. 1

Imprinted methylation acquisition at the (a) Snrpn, (b) Kcnq1ot1, and (c) H19 ICRs in germinal vesicle oocytes was unaltered by increasing maternal age. Each box shows oocytes from spontaneously ovulating females with maternal age in months (mths) (n = 3–4 females per age group). Each line of circles represents the CpGs in the Snrpn, Kcnq1ot1, or H19 ICRs in an individual oocyte (n = 13–19 oocytes per age group). Oocytes were excluded if sequenced DNA clones (n = 5–8/denuded oocyte) exhibited more than one methylation pattern, suggestive of cumulus cell contamination. Black circles, methylated CpGs; white circles, unmethylated CpGs; %5mC, percentage of 5-methyl CpGs over the total number of CpGs for a specific ICR. Same letter (a), no statistically significant difference between groups

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