Fig. 4

Singleplex and multiplex GR bisulfite sequencing in MCF-7 and T47-D cell lines and FFPE cell pellets. GR bisulfite sequencing was performed as a singleplex assay (separate reactions for each primer set) or a multiplex assay (all primer sets used in a single reaction) for cell lines and as a multiplex assay with DNA extracted from formalin-fixed paraffin-embedded cell pellets (Multiplex FFPE). a Heat map of mean methylation levels for each bisulfite sequencing region in the GR promoter. b Heat map of mean methylation level for each CpG contained within GR bisulfite sequencing region A (17 CpGs) and region C (10 CpGs) in T47-D cells