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Fig. 1 | Clinical Epigenetics

Fig. 1

From: Hypermethylation of mismatch repair gene hMSH2 associates with platinum-resistant disease in epithelial ovarian cancer

Fig. 1

Effects of 5-aza-dC treatment on the ovarian cancer cellular sensitivity to cisplatin. a The alterations of the methylation level of hMSH2 promoter in A2780 cells after 5-aza-dC treatment (15 μM) for 72 h. b The change of hMSH2 mRNA expression after treated with the increasing concentrations of 5-aza-dC for 72 h. c, d Western bolt assay showed the increase trend of hMSH2 protein in A2780 cells after treated with the increasing concentrations of 5-aza-dC for 72 h. e CCK-8 assays suggested A2780 cells pre-treated with 5-aza-dC (15 μM) for 72 h showed the obvious decreased proliferation rates to cisplatin compared to control cells (DMSO treatment). f The cell apoptosis rates were significantly increased in A2780 pre-treated with 15 μM 5-aza-dC for 72 h than that in control cells (DMSO treatment) at several cisplatin concentrations by flow cytometry. *P < 0.05; **P < 0.01; ***P < 0.001. Each assay was performed in triplicate

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