Fig. 4

Overview of the differentially methylated regions (DMR) and of their genomic context and box plots of the gene expression levels for the (a) RPS6KB1, (b) ASCL2, and (c) HLA-E genes. The β values of the CpGs that constitute the DMRs are represented by dots. All regions were located 200–1500 bp upstream of the transcriptional start sites. The CpG island track was obtained from the UCSC database. Gene annotation was taken from Illumina reference files according to hg19. Wave 0 was used as the reference. a DMR (4 CpG probes; Stouffer transformation p value = 0.021; adjusted p value for the most significant probe = 4.32 × 10−6; mean β fold change = − 0.018) and the gene expression levels (logFC = − 0.194) of RPS6KB1. b DMR (17 CpG probes; Stouffer transformation p value = 0.598; adjusted p value for the most significant probe = 3.78 × 10−6; mean β fold change = 0.014) and the gene expression levels (logFC = 0.185) of ASCL2. c DMR (5 CpG probes; Stouffer transformation p value = 0.015; adjusted p value for the most significant probe = 1.01 × 10−5; mean β fold change = 0.032) and the gene expression levels (logFC = 0.185) of HLA-E