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Fig. 2 | Clinical Epigenetics

Fig. 2

From: Oral vitamin C supplementation to patients with myeloid cancer on azacitidine treatment: Normalization of plasma vitamin C induces epigenetic changes

Fig. 2

Vitamin C supplement causes increased 5hmC/5mC changes in mononuclear myeloid cells in patients treated with 5-azacytidine. Changes in the global 5hmC/5mC ratio and 5mC levels from baseline (C1D1) to end of study (C3D28) in: a patients receiving vitamin C supplement (500 mg, n = 7) or placebo (n = 9), b patients with (n = 7) or without (n = 9) TET2 mutations, and c DNMTi naïve (n = 9) vs non-naïve (n = 7) patients. Difference between groups is analysed with a Welch two-sample t test. Percentages of global 5hmC and 5mC relative to total levels of deoxyguanosine (dG) were measured using mass spectrometry (LC–MS/MS). The 5hmC/dG level is further related to its substrate, i.e. to the 5mC/dG level (5hmC/5mC)

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