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Fig. 4 | Clinical Epigenetics

Fig. 4

From: Epigenetic down-regulation of the HIST1 locus predicts better prognosis in acute myeloid leukemia with NPM1 mutation

Fig. 4

H3K27me3 HIST1high is associated with a myelomonocytic Gene Expression Profile. a Volcano plot displaying differentially expressed genes between H3K27me3 HIST1high (n = 16) and H3K27me3 HIST1low patients (n = 11). The blue dots represent the histone genes covered by the H3K27me3 mark. b EZH2 and SUZ12 levels of expression obtained from our micro array data were analyzed in H3K27me3 HIST1low (n = 11) and H3K27me3 HIST1high (n = 16) patients. c GSEA (gene set enrichment analysis) of H3K27me3 HIST1high samples. d Expression of three genes associated with granulocytic functions according to H3K27me3 HIST1 status. CYBB (cytochrome B-245 beta chain); FCN1 (Ficolin 1); CLEC4A (C-type lectin domain family 4 member A). Data are represented in relative expression to HPRT. ef Patients from TGCA and GSE 61804 cohorts were separated according to the 3-HIST1 mRNA signature in mRNAlow (n = 114) and mRNAhigh (n = 79) patients. e Myelomonocytic CYBB, FCN1, CLEC4A, ITGAM (integrin subunit alpha M) and f leukemic stem cell SOCS2 (suppressor of cytokine signaling 2), CDK6 (cyclin dependent kinase 6), LAPTM4B (lysosome-associated protein transmembrane-4β), and NGFRAP1 (nerve growth factor receptor-associated protein 1) gene expression was analyzed. Statistical significance was estimated using Mann Whitney test * p < 0.05; **p < 0.005 ***p < 0.0001. NS non-significant

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