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Fig. 1 | Clinical Epigenetics

Fig. 1

From: Epigenetic down-regulation of the HIST1 locus predicts better prognosis in acute myeloid leukemia with NPM1 mutation

Fig. 1

Analysis of H3K27me3 HIST1 level in CN-AML patients. a Heatmap of normalized H3K27me3 enrichment value obtained by H3K27me3 ChIP-qPCR on the indicated HIST1 genes. Enrichment was calculated as the percentage of bound/input and double normalized with HOXD4 and GAPDH. Each column represents a patient sample (n = 46) sorted by unsupervised hierarchical clustering. The separation between H3K27me3 HIST1low and H3K27me3 HIST1high groups is indicated by a dotted lane. Yellow square: mutation for the indicated gene. b Bimodal distribution of the patients according to H3K27me3 HIST1 mean level, obtained from the H3K27me3 enrichment values of the 5 HIST1 regions analyzed. In blue, patients with an H3K27me3 HIST1 mean value below 15 (H3K27me3 HIST1low, n = 21), in red, patients with a mean value above 15 (H3K27me3 HIST1high, n = 25). c OS and EFS in CN-AML patients according to H3K27me3 HIST1low and H3K27me3 HIST1high status

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