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Table 1 Primers and amplification conditions used in the first PCR step for all genes in this study. The capital letters in the primer sequences indicate the original C or G. For all genes, the positions refer to the TSS; for M13mp18, the position refers sequence entry X02513.1 (NCBI, GenBank)

From: Selective demethylation of two CpG sites causes postnatal activation of the Dao gene and consequent removal of d-serine within the mouse cerebellum

Gene

Amplicons

Fw primer

Rv primer

Amplification conditions

Dao (PR1)

+ 3/+ 365

TagTTagagaagtTaggYtgYtYaYta

agattggtgaRRRaaaaaaggagaga

Denature at 95 °C for 2 min; 35 cycles of denaturing at 95 °C for 30 s, annealing at 52 °C for 40 s, and extension at 72 °C for 50 s. Final elongation at 72 °C for 6 m

Dao (PR2)

− 234/+ 137

gaaYagYagTgagYtagYtgg

caccaRccaRRaatRaaacacaa

Denature at 95 °C for 2 min; 38 cycles of denaturing at 95 °C for 30 s, annealing at 54 °C for 40 s, and extension at 72 °C for 50 s. Final elongation at 72 °C for 6 m

Srr

+ 27/+ 377

GTaTtgggagTaaaagTattTag

tttaaactccacaatccaAAcct

Denature at 95 °C for 2 min; 35 cycles of denaturing at 95 °C for 30 s, annealing at 57 °C for 40 s, and extension at 72 °C for 50 s. Final elongation at 72 °C for 6 m

Ddo

− 63/− 468

gtgtgtttTtgaggaggtgaTaTtTa

aActtaccctccattAAtccatAcc

Denature at 95 °C for 2 min; 36 cycles of denaturing at 95 °C for 30 s, annealing at 52 °C for 40 s, and extension at 72 °C for 50 s. Final elongation at 72 °C for 6 m

M13mp18

5946/6294

Ggtgaagggtaattagttgttgtt

ccaataccaaacttacatacct

Denature at 95 °C for 2 min; 33 cycles of denaturing at 95 °C for 30 s, annealing at 57 °C for 40 s, and extension at 72 °C for 50 s. Final elongation at 72 °C for 6 m