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Fig. 7 | Clinical Epigenetics

Fig. 7

From: Deficiency and haploinsufficiency of histone macroH2A1.1 in mice recapitulate hematopoietic defects of human myelodysplastic syndrome

Fig. 7

Effect of macroH2A1.1-GFP transgene overexpression, or macroH2A1.1 KD, on cell proliferation and differentiation. a Histones were extracted from HL-60 cells stably overexpressing a GFP vector or a stable macroH2A1-GFP transgene [9], and lysates were processed for immunoblotting with an anti-GFP antibody. Representative images are shown. b Cell proliferation assay in HL-60 cells, overexpressing GFP or macroH2A1.1-GFP, using labeling with carboxyfluorescein diacetate succinimidyl ester (CFSE) dye dilution by flow cytometry, 4 days upon adding the dye. Data are presented as the percentage of GFP cells, +/− SD, n = 4. **p < 0.01 relative to GFP. c FACS staining for pan-macrophage marker CD11b in HL-60 cells, overexpressing GFP or macroH2A1.1-GFP, upon treatment with Phorbol 12-myristate 13-acetate (PMA, 25 nM) or an equal amount of vehicle (DMSO) for 24 h. Representative gating is shown. d Representative images of HL-60 cells in suspension or adherent upon addition of PMA or DMSO as in c. e Adherent HL-60 cells, overexpressing GFP or macroH2A1.1-GFP, or KD for macroH2A1.1, upon treatment with PMA or DMSO as in c, were counted. Data are presented as the percentage of total cells (in suspension + adherent). Data are presented as means +/− SD, n = 4

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