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Fig. 5 | Clinical Epigenetics

Fig. 5

From: Deficiency and haploinsufficiency of histone macroH2A1.1 in mice recapitulate hematopoietic defects of human myelodysplastic syndrome

Fig. 5

MacroH2A1.1 deficiency decreases rRNA transcription and protein translation in mouse HPCs (a) and in human HL-60 and THP-1 cells (b), respectively. a, b Total RNA was extracted and 47S pre-rRNA levels were measured by qPCR in macroH2A1.1fl/fl versus macroH2A1.1−/− HPCs and in HL-60 or THP-1 control (CTL) cells compared to KD cells for macroH2A1.1, respectively. Data were normalized to the amount of GAPDH mRNA and to the amount of 47S pre-rRNA in macroH2A1.1fl/fl or in CTL cells, respectively. c Global protein synthesis in HL-60 and THP-1 cells treated for 72 h with puromycin (0, 0.5, 2 μγ/ml) was measured by [3H]-leucine incorporation assay and shown as a percentage of inhibition of control in CTL and macroH2A1.1 KD HL-60 and THP-1 cells. n = 3. ***p < 0.001 relative to the respective CTL (0 μg/ml puromycin); #p < 0.05, ###p < 0.001 relative to the respective 0.5 μg/ml puromycin condition

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