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Fig. 3 | Clinical Epigenetics

Fig. 3

From: Deficiency and haploinsufficiency of histone macroH2A1.1 in mice recapitulate hematopoietic defects of human myelodysplastic syndrome

Fig. 3

MacroH2A1.1 deficiency causes myeloid bias upon bone marrow (BM) transplantation. a Peripheral blood (PB) analysis from macroH2A1.1fl/fl, macroH2A1.1fl/− (HET), and macroH2A1.1−/− (KO) mice showing the percentage of myeloid-cell (Mac-1+) and B-cell (B220+) gated populations. b, c Dot plots of PB analysis from animals transplanted with macroH2A1.1fl/fl, macroH2A1.1fl/− (HET), and macroH2A1.1−/− (KO) BM cells in a competitive (b) or noncompetitive (c) transplantation assay. Both plots show the percentage of myeloid cells (Mac-1+) within engrafted CD45.2+ cells. d Representative plots indicating in each quadrant the frequency of gated cell populations within CD45.2+ cells. Graphs show the frequency of [Mac-1+, B (B220+), and T (CD4+/CD8+) cells] within CD45.2+ cells. e Frequencies of CMPs, MEPs, and GMPs in the BM of irradiated macroH2A1.1fl/fl, macroH2A1.1fl/− (HET), and macroH2A1.1−/− (KO) mice. Graphs represent CMP (Linc-Kit+Sca-1CD34+CD16/32), MEP (Linc-Kit+Sca-1CD34CD16/32), and GMP (Linc-Kit+Sca-1CD34+CD16/32+) frequencies in the BM. Results are presented as the means ± SEM of three independent experiments. N = 6–10/group. *p < 0.05, ***p < 0.001, NS = not significant

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