Fig. 2

Assessment of the long-term hematopoietic stem-cell (LT-HSC) pool at a steady state and upon irradiation in macroH2A1.1^fl/− (HET) and macroH2A1.1^−/− (KO) mice. Live, Lin⁻ cells were gated and analyzed for c-Kit and Sca-1 surface marker expression. Lin⁻c-Kit⁺Sca-1⁺ (LSK) cells were gated and further analyzed for CD150⁺ CD48⁻ surface marker expression. Evaluation of the absolute cell number (a) and frequencies (percentage relative to whole bone marrow cell count) (b) of LT-HSCs in the bone marrow (BM) of macroH2A1.1^fl/fl, macroH2A1.1^fl/− (HET), and macroH2A1.1^−/− (KO) mice, at a steady state. N = 5–6 mice per genotype. c, d Evaluation of the absolute cell number and frequencies of LT-HSCs in the BM of irradiated [(600 rad (low dose, c) or 1200 rad (high dose, d)] macroH2A1.1^fl/fl, macroH2A1.1^fl/− (HET), and macroH2A1.1^−/− (KO) mice. Analyses were performed on days 1 (early time point) or 7 (late time point) after irradiation. Error bars represent the SD. N = 3–4 mice per genotype. *p < 0.05, **p < 0.01 relative to macroH2A1.1^fl/fl