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Fig. 4 | Clinical Epigenetics

Fig. 4

From: Co-inhibition of HDAC and MLL-menin interaction targets MLL-rearranged acute myeloid leukemia cells via disruption of DNA damage checkpoint and DNA repair

Fig. 4

Combined treatment with chidamide and MI-3 disrupts DNA damage response. a A heatmap shows hierarchical clustering of four genes, among 635 genes described in Fig. 3a, that were downregulated ≥ 2-folds by chidamide or MI-3 alone and ≥ 4-fold by these two agents in combination. b Real-time PCR analysis was performed to validate the expression of SULT1A3, one of 4 genes shown in panel a, in MOLM-13 cells treated with chidamide ± MI-3 for 48 h. The reaction was carried out in triplicate and relative expression levels were calculated as 2CT after normalization to β-actin. Values indicate mean ± SEM for at least three independent experiments performed in triplicate (**P < 0.01 and ***P < 0.001). c MOLM-13 cells were exposed to chidamide ± MI-3 for 48 h, after which western blot analysis was performed to monitor the expression of the indicated proteins involved in DNA damage checkpoint and DNA repair, as well as γH2A.X, a marker for DNA double-strand breaks

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