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Fig. 3 | Clinical Epigenetics

Fig. 3

From: Promoter methylation of SEPT9 as a potential biomarker for early detection of cervical cancer and its overexpression predicts radioresistance

Fig. 3

SEPT9 affects the biological behavior of cervical cancer cells. a, b Transfected siNC and siSEPT9 in HeLa and transfected NC-LV and SEPT9-LV in CaSki, cell cycle analysis by flow cytometry. c, d Cell viability of Hela and CaSki cells was measured by the CCK8 assay. e, f SEPT9 effects on the invasion and migration of cervical cancer cells with SEPT9 knockdown or overexpression. g Western blot analysis of p-mTOR, p-AKT, cylcinD1, cyclinA2, e-cadherin, and snail in SEPT9 knockdown and overexpression HeLa cells compared to control cells. **P < 0.01, ***P < 0.001. siNC, small interfering RNA negative control; siSEPT9, small interfering RNA targeting SEPT9; NC-LV, pHBLV-CMV-MCS-3flag-EF1-Zsgreen-puro negative control; SEPT9-LV, pHBLV-CMV-MCS-3flag-EF1-Zsgreen-puro tagged SEPT9

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