Fig. 2From: Aberrantly hypermethylated tumor suppressor genes were identified in oral squamous cell carcinoma (OSCC)a–d Epigenetic inactivation of TFPI2, SOX17, GATA4, and FBN2 in OSCC cell lines. The graph displays the expression analysis of four genes after 5-aza-dC treatment. Real-time RT-PCR analysis was carried out to assess four TSG expression levels in OSCC cell lines before and after treatment with 5 μM 5-aza-dC for 72 h. DNA methyltransferase 1 and 3b knockout HCT116 cells (DKO) were included as positive controls. Asterisk indicates a significant increase in gene expression after 5-aza-dC treatment (p < 0.05). The expression levels of genes were internally normalized to the expression levels of GAPDH, and the normalized expression for each gene before 5-aza-dC treatments was set to 1. Gel pictures describe the methylation analysis by methylation-specific PCR (MSP). PCR products recognize unmethylated (U) and methylated (M). DKO cells were used for the unmethylated control. IVD = in vitro methylated control; ddH2O = water control containing no DNABack to article page