Fig. 3

Selected gene expression profile and response to guadecitabine. a Unsupervised hierarchical clustering by baseline expression of the 7 gene panel grouped the phase I patients into two clusters (n = 27). Cluster R patients were clearly resistant to guadecitabine, with CRc seen in 0/9 patients compared to 5/18 patients for cluster S (p = 0.14). b Similar analyses of phase II patients (n = 95). These cluster R patients also showed resistance to guadecitabine (CRc seen in 1/21 patients compared to 22/74 patients for cluster S (p = 0.02), thus confirming the initial data in phase I patients. c A combined analysis of all 122 patients refined the clusters; 27 patients (17.2%) were in cluster R and had lower responses to guadecitabine (CRc seen in 0/27 patients compared to 28/95 patients for cluster S (p = 0.0005)). d Kaplan-Meier survival analysis of all patients by the clusters derived in c. Cluster R (n = 27) had a significantly worse survival after guadecitabine treatment (p = 0.003 by log-rank test)