Rheumatoid arthritis-relevant DNA methylation changes identified in ACPA-positive asymptomatic individuals using methylome capture sequencing

Table 2 Summary of differentially methylated CpGs from different models

Models	Phenotype groups	#CpG tested	#DMCs	#hypoDMCs	#hyperDMCs	#DMRs	#hypoDMRs	#hyperDMRs
I	ACPA-positive vs. ACPA-negative	4,733,057	2047	668	1379	623	210	413
II	ACPA positive vs. ACPA-negative with cell type adjustment	4,635,909	1909	679	1230	509	158	351
III	Self-reported RA vs. ACPA healthy with cell type adjustment	4,109,916	955	435	520	249	81	168
IV	ACPA ordinal (negative/medium/high) with cell type adjustment	4,049,218	4475	869^a	761	1303	62^a	60
V	T cell RA patient vs. controls	3,262,817	1595	868	727	502	202	300

^aThe number of hypo/hyper DMCs/DMRs were calculated based on the directional changes of DNA methylation across ACPA-negative, ACPA-medium-positive, and ACPA-high-positive

ISSN: 1868-7083