Fig. 4
From: The E3 ubiquitin ligase RNF40 suppresses apoptosis in colorectal cancer cells
The loss of RNF40 promotes apoptosis in CRC cells. a HCT116 and b SW837 cells were stained with Annexin V-FITC and propidium iodide and analyzed using the Guava flow cytometry system (Millipore) 72 h after control or RNF40 knockdown. Three independent experiments were performed with three biological replicates per condition each with two technical replicates. The relative number of apoptotic cells was significantly increased in both cell lines after RNF40 knockdown. Mean ± SEM, Student’s t test. c Gated cells of the flow cytometry-based Annexin V assay in HCT116 and d SW837 cells. Red frames indicate gated late apoptotic cells. e Caspase 3/7 activity was measured using a Celigo® S-based ViaStainTM (Nexcelom Bioscience) apoptosis assay and confirmed increased apoptosis after the loss of RNF40 in HCT116 and f SW837 cells. Mean ± SEM, Student’s t test. g Representative images of the ViaStainTM assay indicating DAPI-stained nuclei (blue) and cells with caspase 3/7 activity (green)