Fig. 1

RNF40 loss reduces the tumorigenic properties of CRC cells in vitro. Functional assays were performed using HCT116, HT-29, RKO, SW48, and SW837 cells after siRNA-mediated RNF40 knockdown in three independent experiments. a The morphology of CRC cell lines was not affected 72 h after RNF40 knockdown. Scale bar, 1 mm. b Cellular confluence was measured 72 h post-transfection using a Celigo® S device and decreased cell numbers were detected following RNF40 depletion (n = 6). Mean ± SEM, Students t test. c The clonogenic capacity of CRC cells was determined by quantifying well areas covered by crystal violet-stained colonies formed from single cells (n = 3). Upon loss of RNF40, the cells formed fewer colonies. Mean ± SEM, Students t test. d Representative images of the colony formation assay. e Migration potential was significantly decreased in HCT116 cells with reduced RNF40 levels. Mean ± SEM, Students t test