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Fig. 4 | Clinical Epigenetics

Fig. 4

From: Apabetalone (RVX-208) reduces vascular inflammation in vitro and in CVD patients by a BET-dependent epigenetic mechanism

Fig. 4

In endothelial cells, MZ-1 and apabetalone prevent TNFα induction of key inflammatory and adhesion marker transcripts through the degradation or inhibition of BET proteins respectively. a MZ-1 (1 μM; 6 h) degrades HUVEC BRD2, BRD3, and BRD4 as shown by western blot. b–e TNFα stimulation (2 h) fails to induce HUVEC transcription of VCAM-1 (b), MCP-1 (d), and IL-8 (e) following MZ-1 pretreatment (4 + 2 h). SELE (c) induction is reduced but not eliminated. Apabetalone (20 μM) pretreatment (4 + 2 h) also decreases the level of inductions. Statistical significance was determined through 1-way ANOVA analysis followed by Dunnett’s Multiple Comparison Test using TNFα response for the comparison, where *p < 0.05, **p < 0.01, ***p < 0.001

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