Fig. 1

Differentially methylated loci from IS and IR individuals. a Workflow for processing of DNA methylation data acquired from 450K microarray to generate DMLs. b Heatmap produced from unsupervised hierarchical clustering using the Manhattan distance, complete linkage method displaying DNA methylation (β-value) of the 123 CpGs determined to be DMLs, stratifies IS (green; above column) and IR (purple; above column) from each other. DNA methylation ranges from low (0, blue) to intermediate (0.50, yellow) to high (1.0, red). c Distribution of DMLs in the gene context, with representation of CpGs as lollipops distributed linearly along gene regions indicated (top diagram). Distribution of CpGs along the indicated gene region with expected (orange) and observed (green) frequency over the 450K array shown and compared (left graph). Distribution of CpGs relative to position in CpG islands with expected (orange) and observed (green) frequency over the 450K array shown and compared (right graph). *P < 0.05. d Histogram representation of the distribution of the δ value of IR for all 123 DMLs, as described in methods. Majority of loci were hypomethylated in IR compared to IS